number of copies after pcr?
Posted 13 February 2011 - 05:35 AM
Posted 13 February 2011 - 06:59 AM
You know what happens during a PCR? I suppose you do..
is there a forumla, where if you know the copy# or amount of dna that you add to your pcr reaction, it will calculate how many copies of the dna you will get after pcr?
If you know this, you could calculate it yourself.
Take for example you start with 2 templates (1 piece of dsDNA). How many pieced do you have after 1 cycle? (4).
And after a second cycle ? (8) and a third? (16) ... you a pattern here?
Try to see if you can get to this: Nn=N0X.2n with N0is your start template (how many pieced you have for the primers to attach to), Nnis the number copies of DNA you have, with small n the #of cycles
(sometimes they use this formula: 2n with n =# cycles.... but do you think you can use this all the time?)
and keep in mind: this is in theory...
Edited by pito, 13 February 2011 - 07:06 AM.
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.