number of copies after pcr?
Posted 13 February 2011 - 05:35 AM
Posted 13 February 2011 - 06:59 AM
If you know this, you could calculate it yourself.
Take for example you start with 2 templates (1 piece of dsDNA). How many pieced do you have after 1 cycle? (4).
And after a second cycle ? (8) and a third? (16) ... you a pattern here?
Try to see if you can get to this: Nn=N0X.2n with N0is your start template (how many pieced you have for the primers to attach to), Nnis the number copies of DNA you have, with small n the #of cycles
(sometimes they use this formula: 2n with n =# cycles.... but do you think you can use this all the time?)
and keep in mind: this is in theory...
Edited by pito, 13 February 2011 - 07:06 AM.