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Questions about SNNTE buffer and high salt extract solution


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#1 AllenChiu

AllenChiu

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Posted 12 February 2011 - 10:19 PM

What’s the mechanism of high salt extraction of nuclear/nucleoli protein?
Why would 500mM NaCl be helpful to release proteins?
And one more question,
what is the feature / mechanism of SNNTE wash buffer when it comes to wash steps of IP?
As it seems that people prefer to use SNNTE buffer to do wash steps in their IP which intended to discover new interacting protein.
Is 5% sucrose the major player in it?
Thank you !
PS:what is the original use of SNNTE buffer if you know?




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