Protein purification. How to make function of recombinant protein?
Posted 11 February 2011 - 11:11 AM
p.s. If you have paper, May you tell me too. Thank you for your kind.
Posted 11 February 2011 - 11:46 AM
if not then you can eliminate the imidazole by dialyzing the protein against a more compatible buffer.
if you used urea then you will have to refold the protein by stepwise dialysis to remove urea (and you can remove imidazole at the same time).
genius does what it must
i do what i get paid to do
Posted 12 February 2011 - 07:42 AM
Thank for your suggesion. I use urea ,so i will try stepwise dialize on monday
Edited by mylovesci, 12 February 2011 - 07:46 AM.