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PCR double-bands

Started by Anders, Feb 11 2011 02:32 AM

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#1 Anders

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Posted 11 February 2011 - 02:32 AM

Hi.

Im running some PCRs with bacterial DNA as sample and primers that are "universal" for 16S.
The sample I have should have a fairly low number of bacteria in.

I thought I would just ask the people in here if you have any explanations to these "double-bands" that I get.
Does it indicate several species of bacteria or could it be something else?

/Anders

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#2 hobglobin

Growing old is mandatory, growing up is optional...

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Posted 11 February 2011 - 06:37 AM

Different allels perhaps? Though I'm not sure if bacteria have this...Or different strains or clones that have some difference in this DNA region? Depends what samples you used.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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PCR double-bands

#1 Anders

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#2 hobglobin

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