rpjkmust916, on 22 February 2011 - 04:24 AM, said:
Inmost sun, on 19 February 2011 - 07:03 AM, said:
some might be satisfied with CCB-stained gels to state purity however staining with silver or nanogramm-sensitive dyes is the correct method to state purity on the gel level...
Thanks Inmost sun for your suggestion...unfortunately in my lab only Comassie blue stain available....
while silver stain is very sensitive and qualitative, it is too variable between different proteins to use for quantitation (and, therefore, percent purity). coomassie brilliant blue r-250 (not as variable as g-250) is well suited for this purpose. you may need to load more to see minor contaminants but they are minor and will not significantly influence percent purity determinations
May I ask about the electroelution?
I've read several papers about electroelution...looks like it require some specific apparatus. Is there any simple method that I can use to run electroelution?
Any suggestions, please?
all you need is dialysis tubing, horizontal gel apparatus and a suitable buffer