My lab's recipe for denaturing agarose gel is:
Boric acid 6.85g
Final pH 8.3; final conc=20x
According to the recipe, 1x of this buffer is used as running buffer; to prepare the gel, formaldehyde is added to 1x buffer together with agarose. RNA is loaded to the well with normal dye.
However, i can't find any reference for this method and no one knows where does it comes from. Can anyone verify this method for me?
When i tried to google for denaturing gel, most of the articles i found use MOPS and there is an article using normal TBE buffer (loaded with formamide in well). I dont have MOPS and formamide in my lab. Can i use the method with TBE + formaldehyde? IS there any other choices of recipes?
Edited by hianghao, 10 February 2011 - 05:27 PM.