Mouse Bone Marrow Isolation
Posted 10 February 2011 - 09:44 AM
I'm having trouble isolating cells from the bone marrow of mice. Basically, I think my problem is with cutting the tissue around the leg bones. I use basic scissors to make a small puncture in the leg, and then come in with forceps to tear away the bones. I then cut at the heal and hip with the scissors, followed by more removal of tissue using the forceps. I then stick a needle in the bone to expunge the marrow (25 gauge). Perhaps I should change needle size? Any advice is appreciated.
Posted 10 February 2011 - 07:10 PM
Edited by lab rat, 10 February 2011 - 07:13 PM.