BL-21 Gold (DE3) cells and further strains
Posted 10 February 2011 - 09:09 AM
Posted 10 February 2011 - 09:53 AM
You wrote CAM and TET (tetracycline)? ...why?
Posted 10 February 2011 - 11:15 AM
But BL21 Gold (DE3) is modified version of BL21 cells and resistant to tetracycline. I am little confused now in competence cells preparation and during expression of protein. When cells are resistant to Tetracycline, I can use it inorder to avoid from contemination. But do I need to used Tetracycline during expression of protien from vector which have Amplicilin gene as well.