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Cryopreservation (freezing cells) without using Freezing Container


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#16 citomebo

citomebo

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Posted 15 February 2012 - 06:41 PM

Hi, I didnt know that cells could not be kept above -70. I kept it at -50 to -30 for 2 weeks (because the freezer in my lab cannot decrease the temperature anymore. It just maintain from -30 to -50). After I realized it, I transferred it to liquid nitrogen. Can my cells still be revived? What are the chances? And how can I increase the viability?

By the way, the cell is RAW cells. And I havent thawed it since receiving it from ATCC.

P/S: I am new to cell culture.




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