Hi, I didnt know that cells could not be kept above -70. I kept it at -50 to -30 for 2 weeks (because the freezer in my lab cannot decrease the temperature anymore. It just maintain from -30 to -50). After I realized it, I transferred it to liquid nitrogen. Can my cells still be revived? What are the chances? And how can I increase the viability?
By the way, the cell is RAW cells. And I havent thawed it since receiving it from ATCC.
P/S: I am new to cell culture.
Submit your paper to J Biol Methods today!
Cryopreservation (freezing cells) without using Freezing Container
15 replies to this topic