Odd question. Can I use DNase to decontaminate Primers? Or will that affect PCR later on?
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Odd question. Can I use DNase to decontaminate Primers?
Started by RecklessTech, Feb 09 2011 06:31 AM
2 replies to this topic
#2
lab rat
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Why does a science forum not have pictures of mice and rats?
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Neutral
Neutral
Posted 09 February 2011 - 06:40 AM
I wouldn't advise trying. DNAse I targets pyrimidines, and can attack both double and single-stranded DNA.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.
#3
hobglobin
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Growing old is mandatory, growing up is optional...
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Excellent
Excellent
Posted 09 February 2011 - 08:20 AM
and the removal or deactivation might be a serious problem, as in the PCR you definitely don't want any DNase left...
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
