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Odd question. Can I use DNase to decontaminate Primers?


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#1 RecklessTech

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Posted 09 February 2011 - 06:31 AM

Odd question. Can I use DNase to decontaminate Primers? Or will that affect PCR later on?

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#2 lab rat

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Posted 09 February 2011 - 06:40 AM

I wouldn't advise trying. DNAse I targets pyrimidines, and can attack both double and single-stranded DNA.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 hobglobin

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Posted 09 February 2011 - 08:20 AM

and the removal or deactivation might be a serious problem, as in the PCR you definitely don't want any DNase left...
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.




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