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Antibodies against lyposomes

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#1 Chelo



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Posted 09 February 2011 - 05:07 AM

Dear all,

I would like to raise antibodies against lyposomes (no matter which protein of lipid is the target). I have two questions related to that:

1) do I need to use any kind of adjuvant or just lyposomes alone will be ok? I read somewhere it is possible to add lipid A to the lyposomes to increase their immunogenicity, but I would like to avoid such a cumbersome procedure, if possible.

2) more importanly, is it possible to fix the lyposomes to a plate somehow to titer the antisera by ELISA. Again, here I read there is a complement-dependent lyposome lysis test but I would like to go for ELISA if I had the chance.

Thanks a lot in anticipation.

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