1:7500 dilution with 1 mg/mL stock, now it's 1.25 mg/mL
Posted 09 February 2011 - 12:17 AM
Adding 10 uL to 4.990 mL buffer and then 750uL into 10.5 mL buffer.
The company has changed the stock concentration to 1.25 mg/mL and I want to redo the calculation to ultimately end up at the same final concentration.
I'm not really sure how to do this or where to even start with it. Any suggestions would be welcome.
Posted 09 February 2011 - 03:56 AM
Now the stock is 1250000 ng/ml. So dilute it 1:100 to obtain 12,500 ng/ml then make a 1:93.9 dilution to arrive at 133 ng/ml.
Posted 09 February 2011 - 03:26 PM
So dont use 1Ķl+99Ķl..
And why do you need to do it in 2 steps?
Posted 10 February 2011 - 04:17 AM
Posted 12 February 2011 - 10:11 AM
You are currently adding 10ul of 1mg/ml of OLD stock solution.
New stock solution is 1.25mg/ml
How much new stock solution does one need to add
M1V1 = M2V2
10ul * 1mg/ML = 1.25mg/ml * X volume
X volume = (10ul * 1mg/ML) / 1.25mg/ml = 8ul
Add 8ul of 1.25mg/mL to 4.992 mL buffer and then 750uL into 10.5 mL buffer.
Posted 14 February 2011 - 05:41 PM
Correct, That is why I'm doing it two steps.
Thanks everyone, I'm going to go with Perneseblue's suggestion.