Im trying to figure out how to make 1x PBS+0.3% Triton from the 10% Triton stock solution I have for Immunostaining purposes.
Do I dilute with MilliQ or with PBS?
I would like to make 500 ul of total PBS/0.3% Triton. If someone could help me understand how to dilute from the 10% and whether to use milliQ and/or PBS, I would very much appreciate it!
Thank you so much,
Edited by Biochem_newbie, 08 February 2011 - 09:12 AM.