2 replies to this topic

[baklavas]

Greetings everyone.

   I am on an endeavour to discriminate histochemically amyloid fibers of transthyretin from
amyloid A or κ- and λ- chain amyloid. I have come to realize the routine way
for identifying amyloid - any amyloid - is Congo Red. Further, to discriminate amyloid A
from transthyretin or/and κ-, λ- chains, potassium permanganate is useful: amyloid A
looses the colour while the others retain it.
  
   Wondering just if there is any other way to histochemically differ-diagnose between
all these. F.e., such as any other stain - or, or -ideally - one stain direct for transtyretin.

  Any suggestion based on your experience would be highly welcomed!
  Thanks in advance fellas!

[bob1]

Immunohistochemistry?

[baklavas]

Immunohistochemistry would solve the problem but I dont have access to
specific antibodies - "dyes only" sort of state....