Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Brain tissue homogenization method


  • Please log in to reply
2 replies to this topic

#1 SuRiKaTa

SuRiKaTa

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 February 2011 - 01:19 AM

Hello everybody! I want to isolate total RNA from specific mouse brain regions like striatum and hippocampus (5-50 mg). I would appreciate if you could recommend me a homogenizetion method for the dissected brain tissue. Thanks.

#2 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 236 posts
9
Neutral

Posted 08 February 2011 - 02:38 AM

We use single use disposable teflon homogenization tips. After that, a good way to get rid of the sticky gDNA is to push it through a needle a couple of times.

#3 SuRiKaTa

SuRiKaTa

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 February 2011 - 03:56 AM

BioMiha, thank you for the very quick reply! Can you give me more information about the single use teflon homogenization tips you use (brand, etc.). Is it motor-driven or by hand? Thank you!

Edited by SuRiKaTa, 08 February 2011 - 06:55 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.