Try to knock-down some gene in mice liver using lentiviral packaged shRNA in vivo. Does someone know the detail protocol or literature show the method, such as injection dose, injection method, collection time, et al. Thanks a lot!
Submit your paper to J Biol Methods today!
Anyone know Lentivirus ShRNA in vivo knock down
Started by fuzi, Feb 07 2011 09:02 PM
3 replies to this topic
#2
pcrman
-
- Global Moderators
-
- 1,165 posts
Epigenetist
69
Excellent
Excellent
Posted 15 February 2011 - 09:22 PM
There are published papers on in vivo use of lentiviral delivery of shRNA and you should be able to find some. For example "gene
transfers were performed by injecting 30 μl of lentivirus (titer 2-4*109 TU/ml) encoding shRNAs"
transfers were performed by injecting 30 μl of lentivirus (titer 2-4*109 TU/ml) encoding shRNAs"
#3
scolix
-
- Global Moderators
-
- 314 posts
a student
5
Neutral
Neutral
Posted 16 February 2011 - 10:44 AM
have done it in rodent brain, no idea about liver.
#4
Xavier2
-
- Active Members
-
- 5 posts
member
0
Neutral
Neutral
Posted 12 July 2011 - 10:50 AM
Hi Fuzy,
What don't you try Invivofectamine2.0. It works awesome for delivering siRNA in liver, much easier to prepare than Lentivirus and duration of Knockdown pretty good (>3 weeks after a single injection), just make sure you have a potent and stabe siRNA to start with.
Xavier
What don't you try Invivofectamine2.0. It works awesome for delivering siRNA in liver, much easier to prepare than Lentivirus and duration of Knockdown pretty good (>3 weeks after a single injection), just make sure you have a potent and stabe siRNA to start with.
Xavier
