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293T apoptosis


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#1 CCWW

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Posted 07 February 2011 - 11:02 AM

Hi,

Could anyone tell me a good protocol for the induction of apoptosis in HEK293T cells? Thank you a lot.

#2 bob1

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Posted 07 February 2011 - 05:53 PM

Drugs - tamoxifen, paclitaxel, any of the DNA damage drugs. UV - find a cross linker (e.g. stratalinker) or other UV source with defined output and use that to titrate the amount needed to induce apoptosis in a given time frame.

#3 CCWW

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Posted 07 February 2011 - 06:52 PM

Thank you bob1!!!


Drugs - tamoxifen, paclitaxel, any of the DNA damage drugs. UV - find a cross linker (e.g. stratalinker) or other UV source with defined output and use that to titrate the amount needed to induce apoptosis in a given time frame.






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