I grow CHO (chinese hamster ovary) cells using Ham's F-12 media
Its supplemented with 10% FBS, geneticin, Pen-Strep and 100mM NaOH
I also used 1% Bufferall (buffering agent from Sigma). But now Sigma has stopped its production & say they have no products similar to it
I think bufferall contains HEPES & MOPS but not sure of concentration
Since its a buffering agent, and I think its pretty impt. to have one in the media to maintain pH, i guess i can use any other buffering agent too
Just that i'm not sure what to use. Any ideas?
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Simple & straightforward question
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