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Simple & straightforward question


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#1 Suhas

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Posted 07 February 2011 - 09:07 AM

Hey

I grow CHO (chinese hamster ovary) cells using Ham's F-12 media

Its supplemented with 10% FBS, geneticin, Pen-Strep and 100mM NaOH

I also used 1% Bufferall (buffering agent from Sigma). But now Sigma has stopped its production & say they have no products similar to it

I think bufferall contains HEPES & MOPS but not sure of concentration

Since its a buffering agent, and I think its pretty impt. to have one in the media to maintain pH, i guess i can use any other buffering agent too

Just that i'm not sure what to use. Any ideas?

Suhas

#2 Rnotk

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Posted 07 February 2011 - 04:03 PM

do you need to use that particular recipe for your experiment?

CHO cell is pretty common cell line, so I think you can find another recipe for CHO cell which doesnt require Bufferall.




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