heat inactivation of serum
Posted 07 February 2011 - 05:46 AM
I am working with blood cells, and in this case it is important to inactivate the serum to be used in the culture. My colleague did that at 37°C. Does this ensure the inactivation? I see that many people do it at 56°C for 30 mins.
Thanks in advance
Posted 08 February 2011 - 02:23 PM
Posted 22 February 2011 - 05:28 AM
37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37). 56 deg C for 45 minutes is how I have always done it.
Can u tell me pls why is there a need to inactivate serum ? Sorry for a very basic question.
Posted 22 February 2011 - 04:32 PM