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heat inactivation of serum


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3 replies to this topic

#1 Hussein El Saghire

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Posted 07 February 2011 - 05:46 AM

Hello,

I am working with blood cells, and in this case it is important to inactivate the serum to be used in the culture. My colleague did that at 37C. Does this ensure the inactivation? I see that many people do it at 56C for 30 mins.

Thanks in advance

Hussein

#2 bob1

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Posted 08 February 2011 - 02:23 PM

37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37). 56 deg C for 45 minutes is how I have always done it.

#3 jasmine0507

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Posted 22 February 2011 - 05:28 AM

37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37). 56 deg C for 45 minutes is how I have always done it.


Can u tell me pls why is there a need to inactivate serum ? Sorry for a very basic question.

#4 bob1

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Posted 22 February 2011 - 04:32 PM

It depends on what you are working with, but the serum contains a bunch of stuff from the blood that can cause activation of things like the complement cascade if you are working with immunogen responsive cells such as lymphocytes.




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