3 replies to this topic

[Hussein El Saghire]

Hello,

I am working with blood cells, and in this case it is important to inactivate the serum to be used in the culture. My colleague did that at 37°C. Does this ensure the inactivation? I see that many people do it at 56°C for 30 mins.

Thanks in advance

Hussein

[bob1]

37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37).  56 deg C for 45 minutes is how I have always done it.

[jasmine0507]

bob1, on 08 February 2011 - 02:23 PM, said:

37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37).  56 deg C for 45 minutes is how I have always done it.

Can u tell me pls why is there a need to inactivate serum ? Sorry for a very basic question.

[bob1]

It depends on what you are working with, but the serum contains a bunch of stuff from the blood that can cause activation of things like the complement cascade if you are working with immunogen responsive cells such as lymphocytes.