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elimination of Protein interference from DNA


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#1 rick112

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Posted 06 February 2011 - 10:12 PM

hi
i am trying to detected DNA in a protein solution of high concentration (approx. 10mg/ml) . i am facing accuarcy issue, in terms of over-estimation. the main suspect is Protein.
could you plz suggest me techniques for elimation of this.

thanks a lot

#2 protolder

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Posted 06 February 2011 - 10:51 PM

hi
i am trying to detected DNA in a protein solution of high concentration (approx. 10mg/ml) . i am facing accuarcy issue, in terms of over-estimation. the main suspect is Protein.
could you plz suggest me techniques for elimation of this.

thanks a lot

Hola, you donīt say what type of DNA you want isolate, I suposse genomic, so any of the kits for that with colum washes and elution could help you. Beforehand, extractions with phenol /isoamilic alcohol were used, but in both methods you loose the protein. If you are interested look your DNA purification suppliers or any Mol. Gen. manual. but wait more indications of the forum experts. Buena suerte

#3 bob1

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Posted 07 February 2011 - 05:48 PM

You should be able to remove the protein by chloroform extraction (or by running through a DNA extraction kit). You can't easily estimate DNA content in protein solutions by 260/280 measurement, though you may be able to using a fluorescent based dye.




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