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retrovirus envelope marker


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#1 betheth

betheth

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Posted 04 February 2011 - 10:22 PM

I transected HEK293T cells to produce HIV virus. Now I am testing a way to capture these viruses onto magnetic beads, but not sure if there is a better antigen than the HIV specific antigen. The antibodies to the HIV envelope proteins don't seem have a good affinity. Does anyone know if there is a marker abundant on 293T membrane that package in high copies onto the virus envelope? I used to work with wild HIV and found anti-CD44 immobilize HIV on magnetic beads much more efficiently than anti-gp120 or other HIV antibodies. I wonder if there is a similar surface marker in the 293T produced retroviruses. Thanks a lot:)




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