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[zigi]

Doing a dual luciferase assay with firefly luciferase and renila. What are the reasons that If i am doing a luciferase assay on different days with the same promoter and the same cells I am getting different values of reading F/R =0.1 on one day and F/R =0.5 on the other?

Thank you

Edited by zigi, 04 February 2011 - 08:49 PM.