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uncut plasmid dna vs linearized plasmid gel


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#1 uglearner

uglearner

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Posted 04 February 2011 - 07:00 AM

Hello,

I am going to run a gel comparing my uncut plasmid dna vs linearized plasmid. My insert is 135 bps and my vector is 3Kb. What can I expect to see on my gel, and how many bands can I expect to see. I am assuming the uncut plasmid will have several bands at different sizes and the linearized will have only one, is that right ? I am assuming the total size of my product will now be 3135 bps. Pls advise.

#2 SOS response

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Posted 04 February 2011 - 09:39 AM

Hy,
uncut plasmid will give you only one band high on gel (3135 bp), when you cut you will get two bands - linearized vector (3 kbp) and fragment (135 bps. I dont understand why would uncut plasmid give you several bands?




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