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Help with Isolation of protiens associated with lipid rafts

Started by DrABK, Feb 03 2011 10:32 AM

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#1 DrABK

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Posted 03 February 2011 - 10:32 AM

I am trying to determine if my protein of interest is localized to lipid rafts. I have been using (unsucessfully) a sucrose gradient protocol to do this but was wondering if there are better techniques out there. Does anyone have a lipid raft isolation protocol that consistantly works? Are sucrose gradients the best technique to use? Any help/advice would be greatly appreciated!
Thanks,
ABK

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#2 pDNA

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489 posts

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Posted 03 February 2011 - 12:17 PM

you can find a protocol for the isolation from platlets here ...never did it on my own but i heard that it works fine!

Regards,
p

DrABK, on 03 February 2011 - 10:32 AM, said:

I am trying to determine if my protein of interest is localized to lipid rafts. I have been using (unsucessfully) a sucrose gradient protocol to do this but was wondering if there are better techniques out there. Does anyone have a lipid raft isolation protocol that consistantly works? Are sucrose gradients the best technique to use? Any help/advice would be greatly appreciated!
Thanks,
ABK