Help with Isolation of protiens associated with lipid rafts
Posted 03 February 2011 - 10:32 AM
Posted 03 February 2011 - 12:17 PM
I am trying to determine if my protein of interest is localized to lipid rafts. I have been using (unsucessfully) a sucrose gradient protocol to do this but was wondering if there are better techniques out there. Does anyone have a lipid raft isolation protocol that consistantly works? Are sucrose gradients the best technique to use? Any help/advice would be greatly appreciated!