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Immunoglobulins in serum


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#1 Chelo

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Posted 03 February 2011 - 04:11 AM

Dear all,

I have an hybridoma to produce a monoclonal antibody in flasks using a standard culture medium (RPMI + 10% fetal bovine serum). Because I need this antibody in large quantities, I contacted a private company to mass-produce it. However, I was told that the hybridoma should be growing in serum-free medium. So far, I was assuming that the contribution of cow immunoglobulins to my monoclonals was very low. However, after receiving this warning I realize that I actually donīt have any concrete data to support mu assumption.
Does anybody have data on that? I mean, is it known the concentration of immunoglobulins in the medium I use?
Thank you a lot in advance!

#2 BioMiha

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Posted 03 February 2011 - 06:35 AM

I don't want to advertise anything, however we use these media, so I knew where to look.
http://www.fisher.co...Low_IgG_FBS.pdf
Using serum free medium or low IgG medium usually lowers the specific production of your hybridomas. So it depends on what you need the Ig for. In most applications the cow derived Igs don't affect immunoassays, however some applications require your Igs to be pure, in which case we opt for serum-free media (avoiding the low IgG types).

Edited by BioMiha, 03 February 2011 - 06:35 AM.


#3 Chelo

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Posted 03 February 2011 - 09:16 AM

I don't want to advertise anything, however we use these media, so I knew where to look.
http://www.fisher.co...Low_IgG_FBS.pdf
Using serum free medium or low IgG medium usually lowers the specific production of your hybridomas. So it depends on what you need the Ig for. In most applications the cow derived Igs don't affect immunoassays, however some applications require your Igs to be pure, in which case we opt for serum-free media (avoiding the low IgG types).

Thank you BioMiha -as usual :) - for your kind reply. Beyond the advertising :) , there is a little table with valuable data for me in that pdf file!




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