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Growth curve and coulter counter reading problem?


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#1 Kiana

Kiana

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Posted 02 February 2011 - 04:24 PM

Hi,

I am experiencing some problem for calculating coulter counter reading. I have plated cells in different density of 5000 cells/well, 3000 cells/well ...
In order to use coulter counter usually we add 19.6 ml of Isoton + 0.4 ml cell suspension for the cell counting and finally multiply the reading by 10 power 3.
In this case for plating, as I had two wells for each density and 100 ÁL per well, I have added 19.8 ml Isoton and 0.2 ml of cell sespnesion, I think I need to amend the final reading by some kind of calculation cause the dilutaion factor has been changed.
Can you please let me know how I can calculate the final reading?

Thabk you very much :)




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