Posted 03 May 2002 - 06:48 AM
Posted 03 May 2002 - 07:10 AM
Posted 10 July 2002 - 08:12 AM
1) wash cells 5 x with BSS containing anti-yeast drug (Amphotericin or Nystatin, available from SIGMA Aldrich)
2) Incubate cells with medium plus the drug for 48 hrs.
3) Remove drug to check whether treatment was successful.
Note: this will stress the cells a lot -risk of loosing the culture. Hence don't try it on your only culture.