I've made a cell line in Helas that is Tet inducible. As luck would have it, my flasks became contaminated with yeasts before i had a chance to freeze it down. I don't know where the yeast came from, but it was the only flask contaminated. I've already taken the necessary decontamination steps of hood/incubator. But is there a way to get rid of the yeast from my culture?
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#2
franck
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Posted 03 May 2002 - 07:10 AM
in the air, because yeast can sporulate and spores can stay in aerator filter. Look if you have filter near from culture cells.
#3
Sciad
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Posted 10 July 2002 - 08:12 AM
This is a trouble-some case. What you can try (no guarantee for success):
1) wash cells 5 x with BSS containing anti-yeast drug (Amphotericin or Nystatin, available from SIGMA Aldrich)
2) Incubate cells with medium plus the drug for 48 hrs.
3) Remove drug to check whether treatment was successful.
Note: this will stress the cells a lot -risk of loosing the culture. Hence don't try it on your only culture.
1) wash cells 5 x with BSS containing anti-yeast drug (Amphotericin or Nystatin, available from SIGMA Aldrich)
2) Incubate cells with medium plus the drug for 48 hrs.
3) Remove drug to check whether treatment was successful.
Note: this will stress the cells a lot -risk of loosing the culture. Hence don't try it on your only culture.
