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Run agarose/formaldehyde gel for 5 mins before loading samples? Why?
1 reply to this topic
Posted 02 February 2011 - 04:40 AM
My Molecular Cloning book states that I should run the gel for five minutes at 5 V/cm before switching off and loading the samples. What do you suppose is the purpose of this initial run?
Posted 02 February 2011 - 03:51 PM
I'm guessing that its to equilibrate the gel in the buffer, but I don't know which component is doing the equilibration.