Western for very small protein
Posted 01 February 2011 - 02:03 PM
We did a 15% SDS-PAGE and used a Hybond P PVDF membrane with pore size 0.45micrometer and have done a wet transfer for one hour at 100V. Is it possible the flag-dummy protein just went through the membrane? We can see the 10kDa band of the ladder readily.
Posted 01 February 2011 - 10:56 PM
Posted 02 February 2011 - 11:06 PM