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MTT assay problems


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#1 sushiking

sushiking

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Posted 01 February 2011 - 09:31 AM

hi , i would like to put forward another problem.

during my mtt assay , i seed the wells with 10,000 cell per well of MCF-7 cells. under the microscope , i doesnt seem to be confluent.

but once i run the assay , it give as reading araound 1.0- 1.2 , which is the ideal reading according to a protocol i read from atcc .

so , can anyone explain plse ??? thanks




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