hi , i would like to put forward another problem.
during my mtt assay , i seed the wells with 10,000 cell per well of MCF-7 cells. under the microscope , i doesnt seem to be confluent.
but once i run the assay , it give as reading araound 1.0- 1.2 , which is the ideal reading according to a protocol i read from atcc .
so , can anyone explain plse ??? thanks
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MTT assay problems
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