Pooled RNA from healthy controls
Posted 01 February 2011 - 06:13 AM
Posted 04 February 2011 - 02:02 AM
But be sure to pool each sample with the same concentration. Other thing you should do is run the pool together with each healthy control to see how big variation there is between normal samples. For example if some of them differ more then say 3 Cts from the pooled one, then any result with "diseased" sample closer than 3 Cts is not statistically important, because is within normal range of a healthy sample.
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