Hello all, I wanted to ask you for some ideas about how can I label 2 different cell populations (that are in close proximity). The idea is to maintain the cells in culture for at least 5 days. I have tried some lipophilic tracers, but the component diffuses among cells from both populations. I have also read about fluorescent microspheres, but have never used them. If someone could share with me some ideas/products I would appreciate it a lot, as I am running out of ideas and it would be nice not to have to use transgenes. Thanks!
PS. Sorry my English.
Submit your paper to J Biol Methods today!
Cell labelling
Started by Azifri, Jan 31 2011 05:21 PM
4 replies to this topic
#2
Azifri
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 18 April 2011 - 11:38 AM
Finally I decided to use Qtrackes (if you wanna know) 
#3
roxx_gurl
-
- Active Members
-
- 17 posts
member
3
Neutral
Neutral
Posted 01 July 2014 - 10:59 PM
Hi
I found interesting product of cell labeling dye (PKH 26 and PKH 67, Sigma), which yes tagging on membrane lipid as well. But this article by M.N.M Walter (2010) on wound healing assay is really cool how he tagging selectively on fibroblast and HaCat keratinocytes. But I am still not sure what is the dye tagging on and how they can distinguished both cell. Sigma product sheet not detailed in term of molecular information. I am afraid it will as well co-tagging on my mix population cells, end up no selectively labeling
#4
amelia
-
- Members
-
- 1 posts
member
0
Neutral
Neutral
#5
CPRES
-
- Active Members
-
- 140 posts
Cellcounter
19
Good
Good
Posted 20 January 2015 - 01:30 PM
Here are many live cell imaging products on valdo
So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.
