Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Cell labelling


  • Please log in to reply
6 replies to this topic

#1 Azifri

Azifri

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 31 January 2011 - 05:21 PM

Hello all, I wanted to ask you for some ideas about how can I label 2 different cell populations (that are in close proximity). The idea is to maintain the cells in culture for at least 5 days. I have tried some lipophilic tracers, but the component diffuses among cells from both populations. I have also read about fluorescent microspheres, but have never used them. If someone could share with me some ideas/products I would appreciate it a lot, as I am running out of ideas and it would be nice not to have to use transgenes. Thanks!



PS. Sorry my English.

#2 Azifri

Azifri

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 18 April 2011 - 11:38 AM

Finally I decided to use Qtrackes (if you wanna know) :ph34r:

#3 roxx_gurl

roxx_gurl

    member

  • Active Members
  • Pip
  • 17 posts
3
Neutral

Posted 01 July 2014 - 10:59 PM

Hi

 

I found interesting product of cell labeling dye (PKH 26 and PKH 67, Sigma), which yes tagging on membrane lipid as well. But this article by M.N.M Walter (2010) on wound healing assay is really cool how he tagging selectively on fibroblast and HaCat keratinocytes. But I am still not sure what is the dye tagging on and how they can distinguished both cell. Sigma product sheet not detailed in term of molecular information. I am afraid it will as well co-tagging on my mix population cells, end up no selectively labeling 



#4 amelia

amelia

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 January 2015 - 12:52 AM

lifetechnologies.com this is a site for help



#5 CPRES

CPRES

    Cellcounter

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 140 posts
21
Excellent

Posted 20 January 2015 - 01:30 PM

Here are many live cell imaging products on valdo


So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.


#6 2444256703

2444256703

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 04 March 2015 - 02:22 AM

A http://www.creative-bioarray.com/Services/Cell-Services.htm that may help you 


The secret to happiness is to keep setting yourself new challenges.
 

#7 Andrea Fortina

Andrea Fortina

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
1
Neutral

Posted 23 June 2016 - 09:41 PM

Custom protein labeling & conjugation service provides personalized solutions designed to supply researchers with the widest choice of labels and conjugates to meet their specific needs . We offers high quality, efficient and cost-saving labeling & conjugation services with the largest selections of labels and protocols. We can help you decide on the most suitable conjugate or approach for your applications and optimize labeling procedures toward your specific need. We will find a solution for you at Creative BioMart .

http://www.creativeb...in-Labeling.htm


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.