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Preparation of competent Listeria monocytogenes cells for heat shock tranformati


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#1 lab_microbe

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Posted 31 January 2011 - 02:42 PM

I am trying to find some articles concerning Listeria monocytogenes and heat-shock transformation procedure, but all I came across are only electroporation procedures. At the moment I do not have an access to an electroporator, so I am trying to do it by heat shock. I tried it like for agrobacterium and e.coli, but it did not work, so is anybody familiar with possibility of transforming L.m. in this way, or only electroporation works for L.m.?

I would be really grateful for any suggestions :)

Thanks in advance!




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