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threshold line for RT-pcr

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#1 Xiao Luo

Xiao Luo


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Posted 31 January 2011 - 10:49 AM

Hello everyone,

I have a question about the threshold line and base line. Usually when we run RT-PCR, we just let the iCycler choose the threshold line for me, the problem is whether I need to set the line myself or at what situation , I need to adjust the threshold line and How?

Thank you

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