293 Transfection Troubleshoot !
Posted 30 January 2011 - 01:30 PM
I am doing co-transfection with 293 cells with home-made 'lipofectamine' like protocol.
So I seed them on Day 1, Transfect on Day 2, change the media on Day 3, and lyse them on Day 4.
But after transfection on Day 2, the cells seem to be less viable and Day 3, I don't see them growing compared to just control plate with not transfection.
I seed 750~900000 cells per 10cm dish.... I don't know what the problem is...can somebody help ?
Posted 30 January 2011 - 04:00 PM
Many cells do not do well with transfection as it is, you could try optomising the amount of transfection reagent and/or DNA you are adding.
Posted 01 February 2011 - 04:46 AM