Is it heard of to find secretpry proteins to be localised in the nucleus? My protein of interest is secretory and binds to a receptor on the cell surface but upon IHC, I find it localised in the nuclaus and weak staining in the cytoplasm.
The anibody is polyclonal but gives a right size band on western. Plus, cytoplasmic and nuclear fractions show strong localization in the nuclear fraction upon western.
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