Hey,all
I am dealing with one protein composed by three chains through the disulfide bond. I wonder whether there are some methods to denature and attain each pure chain.
Thanks
Guojun
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1 reply to this topic
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 31 January 2011 - 04:13 PM
i used to use a buffer with 100-200 mM 2-mercaptoethanol. you could also try high concentrations of dithiothreitol.
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genius does what it must
i do what i get paid to do
