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How to attain pure chains of one protein complex


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#1 bio_layman

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Posted 28 January 2011 - 01:23 PM

Hey,all

I am dealing with one protein composed by three chains through the disulfide bond. I wonder whether there are some methods to denature and attain each pure chain.

Thanks

Guojun

#2 mdfenko

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Posted 31 January 2011 - 04:13 PM

i used to use a buffer with 100-200 mM 2-mercaptoethanol. you could also try high concentrations of dithiothreitol.
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