Hey,all
I am dealing with one protein composed by three chains through the disulfide bond. I wonder whether there are some methods to denature and attain each pure chain.
Thanks
Guojun
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 3,259 posts
an elder emeritus
198
Excellent
Excellent
Posted 31 January 2011 - 04:13 PM
i used to use a buffer with 100-200 mM 2-mercaptoethanol. you could also try high concentrations of dithiothreitol.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
