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electroporation/transfection of suspension/myeloid cell lines


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#1 verena.w

verena.w

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Posted 28 January 2011 - 06:56 AM

Hello everybody,

I am trying to electroporate human myeloid cell lines (MV4-11 and Molm-13), unfortunately without succes.
Has anybody some expert knowledge on electroporation of suspension/myeloid cell lines? Maybe a protocol?
Is there any other transfection method you would suggest? Especially for a vector that is 20kb?

Thankx in advance for your anwers!




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