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Dot plot cell cycle analysis-strange dots


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#1 ija0

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Posted 28 January 2011 - 05:33 AM

hi everyone,
i am doing cell cycle analysis via hoechst and pyronin Y. I can distinguish apoptotic cells, G1, G0 and S/G2/M. but I also have dots in my dot plot at higher hoechst intensities than the S/G2/M-population. what is it?
thanks in advance!

#2 Denis Baev

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Posted 29 January 2011 - 12:30 AM

Did you gate-out doublets and aggregates?

#3 ija0

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Posted 29 January 2011 - 01:39 AM

yes, SSC-W vs. SSC-A

#4 Denis Baev

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Posted 31 January 2011 - 03:18 AM

It is not enough.
To precisely gate-out all "strange guys" you have to do the same on FLx-W vs FLx-A (FLx is an FL channel there you detect the DNA dye signal).

Edited by Denis Baev, 31 January 2011 - 03:19 AM.


#5 ija0

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Posted 31 January 2011 - 03:23 AM

ok, than I'll try this. thanks!




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