hi everyone,
i am doing cell cycle analysis via hoechst and pyronin Y. I can distinguish apoptotic cells, G1, G0 and S/G2/M. but I also have dots in my dot plot at higher hoechst intensities than the S/G2/M-population. what is it?
thanks in advance!
0
4 replies to this topic
#2
Denis Baev
-
- Active Members
-
- 53 posts
Enthusiast
0
Neutral
Neutral
Posted 29 January 2011 - 12:30 AM
Did you gate-out doublets and aggregates?
#3
ija0
-
- Active Members
-
- 8 posts
member
0
Neutral
Neutral
Posted 29 January 2011 - 01:39 AM
yes, SSC-W vs. SSC-A
#4
Denis Baev
-
- Active Members
-
- 53 posts
Enthusiast
0
Neutral
Neutral
Posted 31 January 2011 - 03:18 AM
It is not enough.
To precisely gate-out all "strange guys" you have to do the same on FLx-W vs FLx-A (FLx is an FL channel there you detect the DNA dye signal).
To precisely gate-out all "strange guys" you have to do the same on FLx-W vs FLx-A (FLx is an FL channel there you detect the DNA dye signal).
Edited by Denis Baev, 31 January 2011 - 03:19 AM.
#5
ija0
-
- Active Members
-
- 8 posts
member
0
Neutral
Neutral
Posted 31 January 2011 - 03:23 AM
ok, than I'll try this. thanks!
