Dot plot cell cycle analysis-strange dots
Posted 28 January 2011 - 05:33 AM
i am doing cell cycle analysis via hoechst and pyronin Y. I can distinguish apoptotic cells, G1, G0 and S/G2/M. but I also have dots in my dot plot at higher hoechst intensities than the S/G2/M-population. what is it?
thanks in advance!
Posted 31 January 2011 - 03:18 AM
To precisely gate-out all "strange guys" you have to do the same on FLx-W vs FLx-A (FLx is an FL channel there you detect the DNA dye signal).
Edited by Denis Baev, 31 January 2011 - 03:19 AM.