I have a primary cell line derived from fibroblasts. How can I TERT transform them to immortilise them? Is there any kit avaibale for the same? Where can I buy it from? Which companies can do this for me and how much does it cost? Is it possible to do this in the laboratory as well? Kindly elaborate. I stay in England.
Also, can TERT transformation be performed with secondary cell lines too, or is primary cell line prefered?
Please guide.
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bob1
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Thelymitra pulchella
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Posted 28 January 2011 - 11:33 PM
If you have a plasmid with TERT on it and the plasmid contains a eukaryotic selection marker (G418, zeocin, hygromycin, puromycin etc.) then you should be able to do it yourself. It will take about 3-6 months or possibly longer depending on whether you have done stable cell line creation before. You can use this system on any cell line, but ones that are already transformed don't really need to be transformed "more".
