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Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining)


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#1 moljul

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Posted 27 January 2011 - 02:15 AM

Annexin V / Propidium Iodide Staining:

Viable Cells are in the lower left quadrant!
Apoptotic cells are in the lower right quadrant!

Do I have to sum up upper left & right quadrant to determine amount the necrotic cells??? Im not really sure!

thanks for advice

#2 Denis Baev

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Posted 27 January 2011 - 05:55 AM

LL - live
LR- apoptotic (AnnexinV +PI-)
UR - late stage apoptotic (AnnexinV+PI+)
UL - necrotic (AnnexinV- PI+)

#3 moljul

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Posted 28 January 2011 - 12:08 AM

LL - live
LR- apoptotic (AnnexinV +PI-)
UR - late stage apoptotic (AnnexinV+PI+)
UL - necrotic (AnnexinV- PI+)



this was already clear for me before - but can i sum up the upper right (UR) and the upper left (UL) quadrant? I want to do some statistic analysis and graphs for excel!!

Edited by moljul, 28 January 2011 - 12:09 AM.


#4 Denis Baev

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Posted 28 January 2011 - 02:48 AM

Nope. Necrotic cell % is UL only.
The sum of UL and UR % results the total % of dead cells (both necrotic and late stage apoptotic).

#5 BadgerofDoom

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Posted 04 March 2011 - 12:56 AM


LL - live
LR- apoptotic (AnnexinV +PI-)
UR - late stage apoptotic (AnnexinV+PI+)
UL - necrotic (AnnexinV- PI+)



this was already clear for me before - but can i sum up the upper right (UR) and the upper left (UL) quadrant? I want to do some statistic analysis and graphs for excel!!


I think conventionally that the UR quadrent is considered to be either late apoptotic or necrotic i.e. the assay cannot tell the difference. You could sum them both up if you really wanted to since they are both dead but it would probably be better to leave the UL quadrent separate since that is necrotic but not apoptotic so give a bit more information. If you want to tell the difference between late apoptotic and necrotic you may have to look at nuclear morphology or caspases etc.




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