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Isolation of cell membrane


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4 replies to this topic

#1 anonymous

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Posted 04 May 2001 - 09:00 PM

Can anybody recommendate a good method for isolation of cell membrane from cell line? Thanks a lot. Yaoting

#2 anonymous

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Posted 07 June 2001 - 09:00 PM

YES. I AM WORKING ON SIGNALING PATHWAY VIA CELL MEM. AND THE METHOD WE ARE USING IS WORK WELL IN MY HAND.PLEASE LET ME KNOW YOUR EMAIL ADDRESS SO THAT I WILL LIKE TO SEND MY METHOD.

#3 anonymous

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Posted 10 July 2001 - 09:00 PM

Would this method also work for isolating cell surface/membrane associated antigens and if so, could you explain the procedure to me?



#4 anonymous

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Posted 27 July 2001 - 09:00 PM

Hi,Do you think your method will work also for partially purifying receptors from membrane?If so please forward it to me.Yael

#5 anonymous

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Posted 20 August 2001 - 09:00 PM

Would you please give me a protocol to prepare the bacterial membranes for the western blot. The membranes I prepared didn't dissolve properly in Tris buffer.

Thanks, Xueyan Duan





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