Can anybody recommendate a good method for isolation of cell membrane from cell line? Thanks a lot. Yaoting
Isolation of cell membrane
Started by anonymous, May 04 2001 09:00 PM
4 replies to this topic
#1
Posted 04 May 2001 - 09:00 PM
#2
Posted 07 June 2001 - 09:00 PM
YES. I AM WORKING ON SIGNALING PATHWAY VIA CELL MEM. AND THE METHOD WE ARE USING IS WORK WELL IN MY HAND.PLEASE LET ME KNOW YOUR EMAIL ADDRESS SO THAT I WILL LIKE TO SEND MY METHOD.
#3
Posted 10 July 2001 - 09:00 PM
Would this method also work for isolating cell surface/membrane associated antigens and if so, could you explain the procedure to me?
#4
Posted 27 July 2001 - 09:00 PM
Hi,Do you think your method will work also for partially purifying receptors from membrane?If so please forward it to me.Yael
#5
Posted 20 August 2001 - 09:00 PM
Would you please give me a protocol to prepare the bacterial membranes for the western blot. The membranes I prepared didn't dissolve properly in Tris buffer.
Thanks, Xueyan Duan














