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Molarity of DNA for pull down


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#1 kitwai83

kitwai83

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Posted 26 January 2011 - 03:15 AM

Hi all,

I was wondering if anyone knows roughly the minimum number of DNA moles required for a DNA-protein pulldown, in order to get enough protein binding to see it by Western Blot? I guess it depends on the abundance of the protein which is being pulled down however, any idea would be greatly appreciated.

Many thanks.




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