Help for co-transfection of proteins and plasmid
Posted 25 January 2011 - 11:02 AM
I would like to co-transfect recombinant proteins into cells but I also need to transfect the cells with a luciferase reporter plasmid! Has anyone done co-transfection protein-DNA so far? The recombinant protein would be tagged with a fluorescent probe so it would also give me a idea of the transfection efficiency. My main worry would be that usual transfection reagents like metafecten or turbofect can't make the combination protein-DNA. If anyone can help me with that or know a method or a reagent able to do that, that would be great!!
Posted 26 January 2011 - 12:45 AM
Electroporation seems to work for both:
Edited by Rsm, 26 January 2011 - 12:46 AM.