6 replies to this topic

[Boti]

Hello,

Can anyone suggest me what tumor cell line can I use for tumor xenograft model in SV129 background?

Thanks,

Botond

[Fungus_Dreams]

a line specific to the type of cancer you're studying?

[Boti]

a line specific to sv129

I'd like to grow a tumor in sv129 background mice

[Rsm]

A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.

[Boti]

Rsm, on 11 February 2011 - 06:07 AM, said:

A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.

If I'm right. B16 is of mouse origin and not human, right? The same goes for Lewis Lung arcinoma. My problem is that these are black6-derived.

In this case xeno means that the tumor is not of the host animal's origin. But thanks for your input.

Edited by Boti, 13 February 2011 - 02:30 PM.

[Rsm]

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.

[Boti]

Rsm, on 14 February 2011 - 10:18 AM, said:

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.

thank you

I will try that