Xenograft in sv129 mice
Posted 25 January 2011 - 08:42 AM
Can anyone suggest me what tumor cell line can I use for tumor xenograft model in SV129 background?
Posted 28 January 2011 - 03:11 AM
Posted 31 January 2011 - 01:10 AM
I'd like to grow a tumor in sv129 background mice
Posted 11 February 2011 - 06:07 AM
Posted 13 February 2011 - 02:28 PM
A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.
If I'm right. B16 is of mouse origin and not human, right? The same goes for Lewis Lung arcinoma. My problem is that these are black6-derived.
In this case xeno means that the tumor is not of the host animal's origin. But thanks for your input.
Edited by Boti, 13 February 2011 - 02:30 PM.
Posted 14 February 2011 - 10:18 AM
Posted 15 February 2011 - 01:25 PM
Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.
I will try that