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Xenograft in sv129 mice


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#1 Boti

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Posted 25 January 2011 - 08:42 AM

Hello,

Can anyone suggest me what tumor cell line can I use for tumor xenograft model in SV129 background?

Thanks,

Botond

#2 Fungus_Dreams

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Posted 28 January 2011 - 03:11 AM

a line specific to the type of cancer you're studying?

#3 Boti

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Posted 31 January 2011 - 01:10 AM

a line specific to sv129

I'd like to grow a tumor in sv129 background mice

#4 Rsm

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Posted 11 February 2011 - 06:07 AM

A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.
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#5 Boti

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Posted 13 February 2011 - 02:28 PM

A xenograft would be from human to mouse (xeno=foreign), in which case you'll need immunocompromised mouse... For an allograft I would suggest B16 melanoma cells, they are quite efficient in primary and metastatic cancer establishment (but of Bl6 background). Depends on what you're looking for, I guess.


If I'm right. B16 is of mouse origin and not human, right? The same goes for Lewis Lung arcinoma. My problem is that these are black6-derived.

In this case xeno means that the tumor is not of the host animal's origin. But thanks for your input.

Edited by Boti, 13 February 2011 - 02:30 PM.


#6 Rsm

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Posted 14 February 2011 - 10:18 AM

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.
I got soul, but I'm not a soldier

#7 Boti

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Posted 15 February 2011 - 01:25 PM

Try to get some pure Sv129 ES cells. If you inject them, you'll have teratoma formation.


thank you

I will try that




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