Posted 25 January 2011 - 01:24 AM
I´m a PhD student and I´m trying, after some problems, to express a plant protein. Im going to clone in a vector my gene of interest with His-Tag and TEV cleavage site (somepeople tried this way and it works for other specie). My doubt is the next: I know I have to put an ATG start codon before His-Tag but algo my gene of interest has to star with an ATG start codon.
Posted 25 January 2011 - 10:36 PM
Posted 26 January 2011 - 01:09 AM
Thanks for your suggestions.
Firstly, I´ll express in E. coli and then we will see...The option that you mention is a good idea. The background of this lab, the clone a similar protein only with the His-Tag and without the methionine aa from the protein sequence but they didnt use Tev-protease. Gracias por la rápida respuesta .
Posted 26 January 2011 - 01:51 AM