hello, sorry but I am not a biochemistry expert and now am faced with the solubility problem (in theory) of proteins. I have a specific question: if the protein is cleaved at the C-terminal end, will it become more or less soluble without this C-terminal end? at what should I look? pI of the remained protein is around 5 and pI of the C-terminus is 10....does this tell something? please tell me how to deduce solubility here...I have a 3D structure of the protein as well. thank you very much.
Submit your paper to J Biol Methods today!
Solubility of proteins
1 reply to this topic
Posted 26 January 2011 - 10:36 PM
Hola, sometimes tags stabilizes or destabilizes. There are in the net some free tools which predicts the % of probabilities of solubility with the aminoaic sequence. Check the valus for your 2 constructs. Here you are.. www.biotech.ou.edu or www.mips.helmholtz-muenchen.de/proso/proso.seam . Buena suerte