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any idea what can replace this chemical?


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#1 ikwana

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Posted 24 January 2011 - 06:10 PM

i want to isolate allinase from garlic.. n do column chromatography... but, i do not have these chemical..
-pefabloc,PLP,PEG-800, methyl-alpha-D-mannoside, HiLoad 26/60 superdex 200 prep grade,alpha-globulin, racemic alliin,lactate dehydrogenase, NADH


do u have any idea how to replace all these? or how to make it?

#2 Ameya P

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Posted 24 January 2011 - 09:45 PM

i want to isolate allinase from garlic.. n do column chromatography... but, i do not have these chemical..
-pefabloc,PLP,PEG-800, methyl-alpha-D-mannoside, HiLoad 26/60 superdex 200 prep grade,alpha-globulin, racemic alliin,lactate dehydrogenase, NADH


do u have any idea how to replace all these? or how to make it?

ikwana, seems you dont have anything, that is required. Probably, you would be better off buying some kit for the isolation. I dont work with plants, so i cannot recommend any

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#3 genehunter

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Posted 24 January 2011 - 10:54 PM

pefabloc is a protease inhibitor you can substitue with protease inhibitor cocktail.
Not sure what PLP is,
PEG-800 is a readily available chemical,this methyl-alpha-D-mannoside, racemic aniline (??) lactate dehydrogenase, NADH maybe purchased from Sigma.

HiLoad 26/60 superdex 200 prep grade can be substituted with perhaps sephacryl S300
alpha-globulin is a protein standard, you can use BSA, if it is used for quantitation purpose.




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