Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

sphere and suspension culture subculturing


  • Please log in to reply
1 reply to this topic

#1 ija0

ija0

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 24 January 2011 - 01:25 AM

What is definded as a "passage" when subculturing sphere and suspension cultures?
is it only changing the medium after centrifugation or is it a singularization of cells (e.g. by enzymes as trypsin for adherent cultures).
Thanks in advance!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,676 posts
396
Excellent

Posted 24 January 2011 - 02:42 PM

Usually it is when you remove 1/3 or more of the population of cells and start them growing again. I don't know about sphere cultures though.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.